Herpes Handbook

Diagnosis/Testing

Diagnosis

can be made by:

Blood Test


A blood test for herpes is available only in a few research centers.

Microscopic Examination of Tissue Scrapings

In this process, scrapings from lesions are stained and then examined under a microscope.

This test is less accurate than viral cultures, as it is difficult to ascertain which type of HSV infection is present.

One common test is called a Tzanck smear.

Results of the tests take up to 2 weeks.

Viral Cell Culture Test

The test is a specific virus culture or assay for herpes virus.

The doctor or health consultant will obtain fluid from the sores and send it to a laboratory to see if the herpes virus is present.

A viral culture is a fluid sample taken from a lesion when it first appears. Highly accurate results can be given from a culture taken while the lesion is still in the clear blister stage, but the process takes a few days.

The test will not work if the sores have healed.

Immune Response Tests

These tests detect the body's immune response to the herpes virus and are slightly less reliable than viral cultures, but more reliable than tissue scrapings.

Results can sometimes be provided in two hours.

Other available tests:

Antigen test
Pap Smear

Identifying Your Type

Not everyone knows whether they have HSV-1 or HSV-2 and in certain situations that information could be relevant.

In a situation where both partners have HSV-2 both are already infected so precautions to prevent the spread of the disease are optional.

If one partner has genital HSV-1 and the other has HSV-2, each might get infected with a second type unless precautions are used.

HSV- 1

Diagnosing genital HSV- 1 is difficult because the infection seldom recurs. Since many people have HSV-1 orally, a finding of HSV-1 by Western blot serology (blood test) would not positively identify genital infection.

A Western blot confirms if you have HSV-2. If you are seronegative (negative by blood test) for type 2, but positive for type 1, that gives you a strong clue as to the cause of your outbreaks, i.e. seropositive for type 1 but not type 2, with infrequent recurring genital herpes is probably genital HSV1.

Seek immediate professional help if genital herpes is suspected. Some of the available diagnostic procedures become less reliable the longer you wait.

Source: http://www.herpes-coldsores.com/std/herpes.htm

How Herpes Testing Is Done

Herpes Testing

For a viral culture, viral antigen test, or PCR test, a clean cotton swab is rubbed against a herpes sore to collect fluid and cells for examination. Samples may be collected from the vagina, cervix, penis, urethra, eye, throat, or skin.

Health professionals usually collect a sample from small herpes sores that are only a few days old. Viruses are more likely to be found in small newly formed sores.

For an antibody test, the health professional drawing blood will:

Wrap an elastic band around your upper arm to stop the flow of blood. This makes the veins below the band larger so it is easier to put a needle into the vein.

Clean the needle site with alcohol.

Put the needle into the vein. More than one needle stick may be needed.

Attach a tube to the needle to fill it with blood.

Remove the band from your arm when enough blood is collected.

Put a gauze pad or cotton ball over the needle site as the needle is removed.

Put pressure to the site and then a bandage.

What To Think About

Normal test results do not mean you do not have a herpes infection. Herpes is often diagnosed by symptoms and by knowing whether the person has had contact with an infected person. Sometimes a test is not needed.

A person who has genital herpes needs to learn how to avoid spreading the disease, since the disease is more likely to be spread when he or she has sores. If you have recurrent outbreaks, especially during times of stress or illness, you can also spread the disease.

You may want to know whether a herpes infection is due to HSV-1 or HSV-2 so you can take steps to prevent or treat outbreaks.

A genital herpes infection can be spread from a mother to her baby during vaginal delivery. In a newborn, herpes can cause organ failure, brain infection, and death. A pregnant woman with genital herpes will likely have a cervical culture done every week for the last 4 to 6 weeks of the pregnancy before delivery.

If active herpes is present near the time of delivery, a cesarean delivery may be done to prevent infecting the baby.

POCkit is a new test available at some clinics. It checks blood from a finger stick for antibodies to HSV-2. The results are generally ready in about 10 minutes. The POCkit test is more expensive than other tests and may not be available everywhere.

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Herpes Testing

Herpes tests are done to find the herpes simplex virus (HSV). An HSV infection can cause small, painful sores that look like blisters on the skin or the tissue lining mucous membranes of the throat, nose, mouth, urethra, rectum, and vagina.

A herpes infection may cause only a single outbreak of sores, but in many cases the person will have more outbreaks.

There are two types of HSV.

HSV type 1 causes cold sores (also called fever blisters) on the lips. HSV-1 is generally spread by kissing or by sharing eating utensils (such as spoons or forks) when sores are present. HSV-1 can also cause sores around the genitals.

HSV type 2 causes sores in the genital area (genital herpes), such as on or around the vagina or penis. HSV-2 also causes the herpes infection seen in babies who are delivered vaginally in women who have genital herpes. HSV-2 is generally spread by sexual contact. HSV-2 can sometimes cause mouth sores.

In rare cases, HSV can infect other parts of the body, such as the eyes and the brain.

Tests for HSV are most often done only for sores in the genital area. In rare cases, the test may be done using other types of samples, such as spinal fluid, blood, urine, or tears. To see whether sores are caused by HSV, different types of tests may be done.

Herpes viral culture

Cells or fluid from a fresh sore are collected with a cotton swab and placed in a culture cup. A viral culture is the most specific method of finding a genital herpes infection.

Herpes virus antigen detection test

Cells from a fresh sore are scraped off and then smeared onto a microscope slide. This test finds markers (called antigens) on the surface of cells infected with the herpes virus. This test may be done with or in place of a viral culture.

Polymerase chain reaction (PCR) test. A PCR test can be done on cells or fluid from a sore or on blood or on other fluid, such as spinal fluid. PCR finds the genetic material DNA of the HSV virus. This test can tell the difference between HSV-1 and HSV-2. The PCR test is not often done on skin sores, but it is best for testing spinal fluid, for those rare cases in which herpes may cause an infection in or around the brain.

Antibody tests

Blood tests can find antibodies that are made by the immune system to fight a herpes infection. Antibody tests are occasionally done but are not as accurate as a viral culture at finding the cause of a specific sore or ulcer.

Antibody tests cannot tell the difference between a current active herpes infection and a herpes infection that occurred in the past. Because antibodies take time to develop after the first infection, you may not have a positive antibody test if you have just recently been infected. Some blood tests can tell the difference between HSV-1 and HSV-2.

It is thought that about half of adults in the United States likely have herpes antibodies.

A herpes infection cannot be cured. Once you become infected with HSV, the virus stays in the body for life. It "hides" in a certain type of nerve cell and causes more outbreaks of sores in some people.

Recurring infections can be triggered by stress, fatigue, sunlight, or another infection, such as a cold or flu. Medicine can relieve symptoms and shorten the length of the outbreaks, but medicine cannot cure the infection.

A different herpes virus (called varicella zoster) causes chickenpox and shingles.

Why It Is Done

A test for herpes is done to see whether HSV is causing sores, in the genital or mouth area.

How To Prepare

If you may have genital herpes, do not have sexual contact until your test results are back. You can lower the chance of spreading the disease to your partner(s).

If a sample from the urethra will be collected, do not urinate for 2 hours before the test.

For women, if a cervical sample will be collected, do not douche for 24 hours before the test.

How It Is Done -- read in the next post.

To be continued!

http://www.webmd.com/

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